TNF alpha antibody

Catalog Number: GRP45

Product Overview

Product Name	TNF alpha antibody
Catalog Number	GRP45
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat, Bovine
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-P, WB
Immunogen	Full length human TNF alpha Recombinant protein.
Alternative Names	(click to expand) tumor necrosis factor \| DIF \| TNF-alpha \| TNFA \| TNFSF2 \| TNLG1F

Product Properties

Form/Appearance	Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.04 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P01375
Entrez	7124

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000

Validation Images

Non-transfected (â€“) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect

TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibod

TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TNF alpha antibody (GRP497) diluted at 1:400.

TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TNF alpha antibody (GRP497) diluted at 1:400.

TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma . TNF alpha antibody (GRP497) diluted at 1:500.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:500. The HRP-conjugated anti-rabbit Ig

TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis.Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TNF alpha protein stained by TNF alpha antibody (GRP497) diluted at 1:500.Blue: Hoechs

TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. TNF alpha antibody (GRP497) dilution: 1:500.

TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis.Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TNF alpha protein stained by TNF alpha antibody (GRP497) diluted at 1:500.Blue: Hoechst 3

TNF alpha antibody detects TNF alpha protein by western blot analysis.A. 30 μg NCI-H929 whole cell lysate/extract12% SDS-PAGETNF alpha antibody (GRP497) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibod

Untreated (â€“) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GRP497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pr

The WB analysis of TNF alpha antibody was published by Lee AS and colleagues in the journal Cardiovasc Diabetol in 2014.PMID: 24666525

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