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Mre11 antibody [12D7]

Catalog Number: GRP88

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Datasheet
Product Overview
Product Name Mre11 antibody [12D7]
Catalog Number GRP88
Species/Host Mouse
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Tested applications ELISA, FA, ICC, IF, IHC-P, IP, WB
Immunogen Amino acids 182-582 of Mre11 expressed in E. coli.
Alternative Names (click to expand) Mre11 Homolog, Double Strand Break Repair Nuclease | Atld | Hngs1 | Mre11A | Mre11B | Mre11
Product Properties
Form/Appearance Liquid: PBS
Concentration 0.58 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG1
Clonality Monoclonal
Purity Protein G purified
Clone ID 12D7
Uniprot ID P49959
Entrez 4361
Product Description

Mre11 is an 80 kDa protein that acts as a Mn+2 dependent double stranded DNA 3' to 5' exonuclease and a single stranded DNA endonuclease. It is part of a larger complex with Rad50, p95 (Nibrin) and possibly other associated proteins involved in the nucleolytic end-processing of double strand breaks and telomere maintenance.

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000
Validation Images
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GRP540) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was develo
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GRP540) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was develo
Mre11 antibody [12D7] detects Mre11 protein by western blot analysis.A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human Mre11-transfected 293T cells7.5% SDS-PAGEMre11 antibody [12D7] (GRP540) dilution: 1:1000The HRP-conjugated ant
Mre11 antibody [12D7] detects Mre11 protein by western blot analysis.A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human Mre11-transfected 293T cells7.5% SDS-PAGEMre11 antibody [12D7] (GRP540) dilution: 1:1000The HRP-conjugated ant
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GRP540) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (GRP540) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Mre11 stained by Mre11 antibody [12D7] (GRP540) diluted at 1:200.Blue: Hoechst 33342 staining
Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Mre11 stained by Mre11 antibody [12D7] (GRP540) diluted at 1:200.Blue: Hoechst 33342 staining
The WB analysis of Mre11 antibody [12D7] was published by Harten SK and colleagues in the journal BMC Biol in 2015.PMID: 25857663
The WB analysis of Mre11 antibody [12D7] was published by Harten SK and colleagues in the journal BMC Biol in 2015.PMID: 25857663
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