IL1 beta antibody

Product Overview

Product Name	IL1 beta antibody
Catalog Number	GRP177
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ELISA, FA, ICC, IF, IHC-Fr, IHC-P, WB
Immunogen	Recombinant murine IL1 beta
Alternative Names	(click to expand) interleukin 1 beta \| IL-1beta \| Il-1b

Product Properties

Form/Appearance	Liquid: PBS
Concentration	1.3 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P10749
Entrez	16176

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,ELISA: 0.5-2?g/ml

Validation Images

An ELISA plate is coated with 50 μL of IL1 beta recombinant protein at concentration of 5 μg/mL. The coated protein is detected with anti-IL1 beta antibody (GRP629) at concentration ranged from 0.01 to 0.15 μg/mL.

Untreated (â€“) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GRP629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pri

IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.Sample: Frozen-sectioned mouse hippocampus.Green: IL1 beta stained by IL1 beta antibody (GRP629) diluted at 1:250.Blue: Fluoroshield with DAPI.

Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GRP629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Untreated (â€“) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GRP629) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used

Untreated (â€“) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GRP629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pri

IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL1 beta stained by IL1 beta antibody (GRP629) diluted at 1:500.

IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: IL1 beta stained by IL1 beta antibody (GRP629) diluted at 1:250.Red: NF-H, a neuron marker, stained by NF-H antibody [G

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IL1 beta antibody

Catalog Number: GRP177