Availability
- Request Lead Time
- In stock and ready for quick dispatch
Product Overview
Product Name | Anti-HLA-G Purified |
---|---|
Catalog Number | GRP10596 |
Reactivity | Human |
Tested applications | ELISA, FC, ICC, IHC-Fr, IHC-P, WB |
Immunogen | C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin. |
Product Properties
Concentration | 1 mg/ml |
---|---|
Storage | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. |
Note | For research use only. |
Isotype | Mouse IgG1 |
Clonality | Monoclonal |
Clone ID | 5A6G7 |
Uniprot ID | P17693 |
NCBI | 3135 |
Product Description
The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Application Notes
Application Notes | Immunohistochemistry (frozen sections): recommended dilution: 10 μg/ml, positive tissue: placenta Flow cytometry: recommended dilution: 2 μg/ml Immunohistochemistry (paraffin sections): recommended dilution: 10 μg/ml, positive tissue: placenta ELISA: positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant, negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100). Western blotting: positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE |
---|
Reviews
Write Your Own Review