SQSTM1 / P62 antibody [N3C1], Internal

Catalog Number: GRP15

Product Overview

Product Name	SQSTM1 / P62 antibody [N3C1], Internal
Catalog Number	GRP15
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat, Bovine, Zebrafish, Honeybee
Conjugation	Unconjugated
Tested applications	FACS, ICC, IF, IHC-P, IP, WB
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human SQSTM1 / P62. The exact sequence is proprietary.
Alternative Names	(click to expand) sequestosome 1 \| A170 \| DMRV \| FTDALS3 \| NADGP \| OSIL \| PDB3 \| ZIP3 \| p60 \| p62 \| p62B

Product Properties

Form/Appearance	Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.29 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	Q13501
Entrez	8878

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,FACS: 1:50-1:200,IP: 1:100-1:500

Validation Images

SQSTM1 antibody [N3C1], Internal (GRP467) detects SQSTM1 protein by flow cytometry analysis.Sample: HeLa cell fixed in 4% paraformaldehyde at 4ÂºC for 5 min.Brown: Unlabelled sample was also used as a control.Blue: SQSTM1 antibody [N3C1], Internal] dilut

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50?M Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: SQSTM1 pr

Untreated (â€“) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GRP467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to de

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract10% SDS-PAGESQSTM1 antibody [N3C1], Internal (GRP467) dilution: 1:1000 The HRP-conjugate

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.Samples: HepG2 cells treated with 3?M thapsigargin 12 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acet

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.A. 30 μg NIH-3T3 whole cell lysate/extract B. 30 μg JC whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract 12% SDS-PAGESQSTM1 antibody [N3C1], Interna

Untreated (â€“) and treated (+) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GRP467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to de

SQSTM1 / P62 antibody [N3C1], Internal detects SQSTM1 / P62 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.SQSTM1 / P62 stained by SQSTM1 / P62 antibody [N3C1], Internal (GRP467) diluted at 1:500.Antigen R

Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 ?g of SQSTM1 antibody [N3C1], Internal (GRP467).Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal (GRP467).EasyBlot anti-Rabbit IgG was used as a seconda

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