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SOX2 antibody [N1C3]

Catalog Number: GRP105

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Datasheet
Product Overview
Product Name SOX2 antibody [N1C3]
Catalog Number GRP105
Species/Host Rabbit
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Tested applications FACS, ICC, IF, IHC-Fr, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
Alternative Names (click to expand) SRY-box 2 | ANOP3 | MCOPS3
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.15 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P48431
Entrez 6657
Product Description

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Application Notes
Dilution Range WB: 1:5000-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,FACS: 1:50-1:200,IP: 1:100-1:500
Validation Images
SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GRP557) at a dilution of 1:2500.
SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GRP557) at a dilution of 1:2500.
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GRP557) antibody at 1:80 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GRP557) antibody at 1:80 dilution.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. SOX2 antibody [N1C3] (GRP557) dilution: 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. SOX2 antibody [N1C3] (GRP557) dilution: 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. SOX2 antibody [N1C3] (GRP557) diluted at 1:500.
SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GRP557) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used
SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GRP557) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used
SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Blue: Fluoroshield with DAPI.
SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Blue: Fluoroshield with DAPI.
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GRP557) antibody at 1:160 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GRP557) antibody at 1:160 dilution.
Flow cytometry on human embryonic stem cells, staining with SOX2 (GRP557)antibody at 1:100 dilution(purple) or rabbit IgG (black).
Flow cytometry on human embryonic stem cells, staining with SOX2 (GRP557)antibody at 1:100 dilution(purple) or rabbit IgG (black).
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluo
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluo
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GRP557) antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GRP557) antibody at 1:100 dilution.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:500.Red: phalloidin, a cytoskeleton
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:500.Red: phalloidin, a cytoskeleton
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GRP557) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GRP557) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The ICC/IF analysis of SOX2 antibody [N1C3] was published by Chang WF and colleagues in the journal PLoS One in 2016.PMID: 27802323
The ICC/IF analysis of SOX2 antibody [N1C3] was published by Chang WF and colleagues in the journal PLoS One in 2016.PMID: 27802323
The WB analysis of SOX2 antibody [N1C3] was published by Misuno K and colleagues in the journal Stem Cell Res Ther in 2013.PMID: 24423398
The WB analysis of SOX2 antibody [N1C3] was published by Misuno K and colleagues in the journal Stem Cell Res Ther in 2013.PMID: 24423398
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 ?g of SOX2 antibody [N1C3] (GRP557).Western blot analysis was performed using SOX2 antibody [N1C3] (GRP557).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 ?g of SOX2 antibody [N1C3] (GRP557).Western blot analysis was performed using SOX2 antibody [N1C3] (GRP557).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fetal brain. Green: SOX2 antibody [N1C3] (GRP557) diluted at 1:200. The signal was developed using goat anti-rabbit
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fetal brain. Green: SOX2 antibody [N1C3] (GRP557) diluted at 1:200. The signal was developed using goat anti-rabbit
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 ?g of SOX2 antibody [N1C3] (GRP557) or SOX2 antibody [GT1876] (GRP557).Western blot analysis was performed using SOX2 antibody [N1C3] diluted at 1:500.EasyBlot anti-Rabbit IgG was
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 ?g of SOX2 antibody [N1C3] (GRP557) or SOX2 antibody [GT1876] (GRP557).Western blot analysis was performed using SOX2 antibody [N1C3] diluted at 1:500.EasyBlot anti-Rabbit IgG was
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker,
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GRP557) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker,
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