Iba1 antibody [GT10312]

Catalog Number: GRP175

Product Overview

Product Name	Iba1 antibody [GT10312]
Catalog Number	GRP175
Species/Host	Mouse
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	FACS, ICC, IF, IHC-Fr, IHC-P, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.
Alternative Names	(click to expand) allograft inflammatory factor 1 \| AIF-1 \| IBA1 \| IRT-1 \| IRT1

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7)
Concentration	1.41 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG1
Clonality	Monoclonal
Purity	Affinity purified by Protein G.
Clone ID	GT10312
Uniprot ID	P55008
Entrez	199

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,FACS: 1:50-1:200

Validation Images

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GRP627) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 protein stained by Iba1 antibody [GT10312] (GRP627) diluted at 1:200.Blue: Hoechst 3

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GRP627) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was devel

Iba1 antibody [GT10312] detects Iba1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse brain.Green: Iba1 stained by Iba1 antibody [GT10312] (GRP627) diluted at 1:200.Blue: Hoechst 33342 staining.

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Iba1 stained by Iba1 antibody [GT10312] (GRP627) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Iba1 stained by Iba1 antibody [GT10312] (GRP627) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody [GT10312] (GRP627) detects AIF1 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: Iba1 antibody [GT10312] (GRP627) dilution: 1:50. Acquisition of 20,000 events were collected us

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