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Product Overview
| Product Name | Heme Oxygenase 1 antibody |
|---|---|
| Catalog Number | GRP19 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Monkey |
| Conjugation | Unconjugated |
| Tested applications | ELISA, ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.99 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P09601 |
| Entrez | 3162 |
Product Description
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human renal carcinoma . Heme Oxygenase 1 antibody (GRP471) diluted at 1:500.
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GRP471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GRP471) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 an
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GRP471) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the
Heme Oxygenase 1 antibody detects HMOX1 protein at cytoplasm in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Heme Oxygenase 1 antibody (GRP471) diluted at 1:500.
Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using GRP471 as capture antibody at concentration of 5 μg/mL and as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and u
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