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Rabbit anti-Goat IgG (H&L), HRP conjugated - trial sample
Catalog Number: GRP12237
Product Overview
| Product Name | Rabbit anti-Goat IgG (H&L), HRP conjugated - trial sample |
|---|---|
| Catalog Number | GRP12237 |
| Species/Host | Rabbit |
| Reactivity | Goat |
| Predicted Reactivity | Goat IgG Heavy and Light chains (H&L) |
| Tested applications | ELISA, WB, IHC |
| Immunogen | purified goat IgG, whole molecule |
Product Properties
| Form/Appearance | Liquid |
|---|---|
| Storage | Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Purified rabbit IgG |
Application Notes
| Dilution Range | 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000 - 50 000 (WB) |
|---|---|
| Application Notes | Additional Information: No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Background: Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays. |
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