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C3 antibody [C3], C-term

Catalog Number: GRP102

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Datasheet
Product Overview
Product Name C3 antibody [C3], C-term
Catalog Number GRP102
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.
Alternative Names (click to expand) complement C3 | AHUS5 | ARMD9 | ASP | C3a | C3b | CPAMD1 | HEL-S-62p
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.08 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P01024
Entrez 718
Product Description

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term (GRP554) diluted at 1:500.
C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term (GRP554) diluted at 1:500.
C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C3 protein stained by C3 antibody [C3], C-term (GRP554) diluted at 1:200.Blue: Hoechst 3334
C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C3 protein stained by C3 antibody [C3], C-term (GRP554) diluted at 1:200.Blue: Hoechst 3334
Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody (GRP554) diluted at 1:1000.
Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody (GRP554) diluted at 1:1000.
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GRP554) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GRP554) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GRP554) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GRP554) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ?g of C3 antibody [C3], C-term (GRP554).Western blot analysis was performed using C3 antibody [C3], C-term (GRP554).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 ?g of C3 antibody [C3], C-term (GRP554).Western blot analysis was performed using C3 antibody [C3], C-term (GRP554).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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