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BRCA1 antibody [17F8] - ChIP grade

Catalog Number: GRP85

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Datasheet
Product Overview
Product Name BRCA1 antibody [17F8] - ChIP grade
Catalog Number GRP85
Species/Host Mouse
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ChIP, ELISA, ICC, IF, IHC-P, IP, WB
Immunogen Protein fragment expressed in E. coli corresponding to amino acids 762-1315.
Alternative Names (click to expand) Brca1, Dna Repair Associated | Brcai | Brcc1 | Brovca1 | Fancs | Iris | Pnca4 | Ppp1R53 | Pscp | Rnf53 | Brca1
Product Properties
Form/Appearance Liquid: PBS
Concentration 1.55 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG1
Clonality Monoclonal
Purity Protein G purified
Clone ID 17F8
Uniprot ID P38398
Entrez 672
Product Description

BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the Aexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1 ?g/ml
Validation Images
BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GRP537) diluted by 1:500. The HRP-conjugated anti
BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GRP537) diluted by 1:500. The HRP-conjugated anti
The IHC-P analysis of BRCA1 antibody [17F8] - ChIP grade was published by Bernard-Gallon DJ and colleagues in the journal Breast Cancer Res in 2001.PMID: 11250747
The IHC-P analysis of BRCA1 antibody [17F8] - ChIP grade was published by Bernard-Gallon DJ and colleagues in the journal Breast Cancer Res in 2001.PMID: 11250747
The IHC-P analysis of BRCA1 antibody [17F8] - ChIP grade was published by Bernard-Gallon DJ and colleagues in the journal Breast Cancer Res in 2001.PMID: 11250747
The IHC-P analysis of BRCA1 antibody [17F8] - ChIP grade was published by Bernard-Gallon DJ and colleagues in the journal Breast Cancer Res in 2001.PMID: 11250747
BRCA1 antibody 6B4 (GRP537) and BRCA1 antibody 17F8 (GRP537) was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 c
BRCA1 antibody 6B4 (GRP537) and BRCA1 antibody 17F8 (GRP537) was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 c
BRCA1 antibody 6B4 (GRP537) and BRCA1 antibody 17F8 (GRP537) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enric
BRCA1 antibody 6B4 (GRP537) and BRCA1 antibody 17F8 (GRP537) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enric
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