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Bcl-2 antibody [N1N2], N-term

Catalog Number: GRP3

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Datasheet
Product Overview
Product Name Bcl-2 antibody [N1N2], N-term
Catalog Number GRP3
Species/Host Rabbit
Reactivity Human, Mouse, Rat, Zebrafish
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, WB
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.
Alternative Names (click to expand) BCL2 apoptosis regulator | Bcl-2 | PPP1R50
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.48 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P10415
Entrez 596
Product Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000
Validation Images
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Bcl-2 stained by Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:5
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Bcl-2 stained by Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:5
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GRP455) antibody at 1:250 dilution.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GRP455) antibody at 1:250 dilution.
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:500.
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:500.
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresp
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GRP455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresp
The WB analysis of Bcl-2 antibody [N1N2] was published by Li CJ and colleagues in the journal Evid Based Complement Alternat Med in 2012.PMID: 23091557
The WB analysis of Bcl-2 antibody [N1N2] was published by Li CJ and colleagues in the journal Evid Based Complement Alternat Med in 2012.PMID: 23091557
The WB analysis of Bcl-2 antibody [N1N2] was published by Wen Y and colleagues in the journal Oxid Med Cell Longev in 2019.PMID: 31210840
The WB analysis of Bcl-2 antibody [N1N2] was published by Wen Y and colleagues in the journal Oxid Med Cell Longev in 2019.PMID: 31210840
The WB analysis of Bcl-2 antibody [N1N2] was published by Fu X and colleagues in the journal PLoS One in 2016.PMID: 26859149
The WB analysis of Bcl-2 antibody [N1N2] was published by Fu X and colleagues in the journal PLoS One in 2016.PMID: 26859149
The WB analysis of Bcl-2 antibody [N1N2], N-term was published by Hsu WH and colleagues in the journal PLoS One in 2015.PMID: 25849560
The WB analysis of Bcl-2 antibody [N1N2], N-term was published by Hsu WH and colleagues in the journal PLoS One in 2015.PMID: 25849560
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