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Product Overview
| Product Name | ATR (phospho Thr1989) antibody |
|---|---|
| Catalog Number | GRP70 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, IP, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human ATR (phospho Thr1989). The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.57 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q13535 |
| Entrez | 545 |
Product Description
The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. ATR (phospho Thr1989) antibody (GRP522) diluted at 1:250.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ATR (phospho Thr1989) antibody (GRP522) diluted at 1:250.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) antibody (GRP522) diluted at 1:200.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) antibody (GRP522) diluted at 1:200.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GRP522) diluted at 1:500.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GRP522) diluted at 1:500.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATR (phospho Thr1989) stained by ATR (phospho Thr1989) antibody (GRP
ATR (phospho Thr1989) antibody detects ATR protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. ATR (phospho Thr1989) antibody (GRP522) diluted at 1:250.
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. ATR (phospho Thr1989) antibody (GRP522) diluted at 1:250.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GRP522) diluted at 1:2000.
Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GRP522) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to det
Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GRP522) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody (GRP522) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect
Immunoprecipitation of ATR (phospho Thr1989) protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 ?g of ATR (phospho Thr1989) antibody (GRP522) antibody (GRP522).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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