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ATP6V1A antibody

Catalog Number: GRP129

Product Overview
Product Name ATP6V1A antibody
Catalog Number GRP129
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.57 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P38606
Entrez 523
Product Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:1000-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
ATP6V1A antibody detects ATP6V1A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GRP581) diluted by 1:10000. The HRP-conjugated anti-rabbit IgG a
Sample (20 μg of whole cell lysate) A: mouse brain 7.5% SDS PAGE GRP581 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunofluorescence analysis of methanol-fixed A431, using ATP6V1A(GRP581) antibody at 1:500 dilution.
ATP6V1A antibody detects ATP6V1A protein at cytoplasm on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ATP6V1A antibody (GRP581) diluted at 1:500.
Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 ?g of ATP6V1A antibody (GRP581).Western blot analysis was performed using ATP6V1A antibody (GRP581).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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