Availability
- Request Lead Time
- In stock and ready for quick dispatch
- Usually dispatched within 5-10 working days
Product Overview
| Product Name | ATG5 antibody |
|---|---|
| Catalog Number | GRP58 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q9H1Y0 |
| Entrez | 9474 |
Product Description
ATG5 antibody
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50?M Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATG5 protein stained by ATG5 a
ATG5 antibody detects ATG5 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ATG5 antibody (GRP510) diluted at 1:400.
Sample (30 μg of whole cell lysate) A: NT2D1 B: PC-3 12% SDS PAGE GRP510 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis.Sample: HepG2 cells treated with 3?M thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for
ATG5 antibody detects ATG5 protein by western blot analysis. Un-treated (-) and treated (+, 50 ?M chloroquine treatment for 24 hrs) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATG5 antibody (GRP510
Reviews
Write Your Own Review
