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Product Overview
| Product Name | ATG12 antibody |
|---|---|
| Catalog Number | GRP62 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.86 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | O94817 |
| Entrez | 9140 |
Product Description
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Sample (50 μg of whole cell lysate) A: Rat brain 12% SDS PAGE GRP514 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A: NIH-3T3 12% SDS PAGE GRP514 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ATG12 antibody detects ATG12 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ATG12 antibody (GRP514) diluted at 1:500.
ATG12 antibody detects ATG12 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.ATG12 stained by ATG12 antibody (GRP514) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GRP514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 ?g of ATG12 antibody (GRP514).Western blot analysis was performed using ATG12 antibody (GRP514) diluted at 1:1000.EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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