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Product Overview
| Product Name | Ataxin 3 antibody |
|---|---|
| Catalog Number | GRP145 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ataxin 3. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.35 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P54252 |
| Entrez | 4287 |
Product Description
Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and blotted with Ataxin 3 antibody (GRP597) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to det
Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis.293T mock transfected (-) or transfected with a construct expressing human Ataxin 3 (+).10% SDS-PAGEAtaxin 3 antibody (GRP597) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Ataxin 3(GRP597) antibody at 1:500 dilution.
Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ataxin 3 protein stained by Ataxin 3 antibody (GRP597) diluted at 1:250.Blue:
Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ataxin 3 stained by Ataxin 3 antibody (GRP597) diluted at 1:500.Blue: Hoechst 33
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GRP597) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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