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Product Overview
| Product Name | ASCL1 antibody |
|---|---|
| Catalog Number | GRP156 |
| Species/Host | Rabbit |
| Reactivity | Human, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ASCL1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P50553 |
| Entrez | 429 |
Product Description
This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Various whole cell and SH-SY5Y nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GRP608) diluted at 1:1000.
ASCL1 antibody detects ASCL1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ASCL1 antibody (GRP608) diluted at 1:500.
ASCL1 antibody detects ASCL1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15 % SDS-PAGE, and blotted with ASCL1 antibody (GRP608) diluted by 1:1000
SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GRP608) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GRP608) diluted at 1:1000.
ASCL1 antibody detects ASCL1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ASCL1 stained by ASCL1 antibody (GRP608) diluted at 1:500.Red: beta Tubulin
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