Apolipoprotein E antibody [C2C3], C-term

Catalog Number: GRP94

Product Overview

Product Name	Apolipoprotein E antibody [C2C3], C-term
Catalog Number	GRP94
Species/Host	Rabbit
Reactivity	Human
Conjugation	Unconjugated
Tested applications	ELISA, ICC, IF, IHC-P, IP, WB
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.
Alternative Names	(click to expand) apolipoprotein E \| AD2 \| APO-E \| ApoE4 \| LDLCQ5 \| LPG

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.66 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P02649
Entrez	348

Product Description

Application Notes

Dilution Range	WB: 1:500-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500,ELISA: 1:1000-1:10000

Validation Images

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-t

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3]

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GRP546) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.IP Sample: HepG2 whole cell lysate/extractA : 30 ?g whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cellsB : Control with 3 ?g of

Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.A. 30 μg HepG2 whole cell lysate/extract12% SDS-PAGEApolipoprotein E antibody [C2C3], C-term (GRP546) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody w

Sample (2.5 μg of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE GRP546 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was u

Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using GRP546 as capture antibody at concentration of 5 μg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 μg/mL. Mouse

Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GRP546) diluted at 1:50

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