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Apolipoprotein E antibody [C2C3], C-term

Catalog Number: GRP94

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Datasheet
Product Overview
Product Name Apolipoprotein E antibody [C2C3], C-term
Catalog Number GRP94
Species/Host Rabbit
Reactivity Human
Conjugation Unconjugated
Tested applications ELISA, ICC, IF, IHC-P, IP, WB
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.
Alternative Names (click to expand) apolipoprotein E | AD2 | APO-E | ApoE4 | LDLCQ5 | LPG
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.66 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P02649
Entrez 348
Product Description

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500,ELISA: 1:1000-1:10000
Validation Images
Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-t
Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-t
Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3]
Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3]
Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GRP546) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GRP546) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.IP Sample: HepG2 whole cell lysate/extractA : 30 ?g whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cellsB : Control with 3 ?g of
Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.IP Sample: HepG2 whole cell lysate/extractA : 30 ?g whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cellsB : Control with 3 ?g of
Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.A. 30 μg HepG2 whole cell lysate/extract12% SDS-PAGEApolipoprotein E antibody [C2C3], C-term (GRP546) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody w
Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.A. 30 μg HepG2 whole cell lysate/extract12% SDS-PAGEApolipoprotein E antibody [C2C3], C-term (GRP546) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody w
Sample (2.5 μg of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE GRP546 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was u
Sample (2.5 μg of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE GRP546 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was u
Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using GRP546 as capture antibody at concentration of 5 μg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 μg/mL. Mouse
Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using GRP546 as capture antibody at concentration of 5 μg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 μg/mL. Mouse
Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GRP546) diluted at 1:50
Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GRP546) diluted at 1:50
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