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Product Overview
| Product Name | Apolipoprotein A1 antibody |
|---|---|
| Catalog Number | GRP51 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Full length human Apolipoprotein A1 Recombinant protein. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.64 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P02647 |
| Entrez | 335 |
Product Description
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GRP503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GRP503) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GRP503) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GRP503) diluted
Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GRP503) diluted at 1:500.Antigen Retrieval: Citra
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