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Anti-SARS-CoV-2 Nucleocapsid (N) Protein (RABBIT) Antibody
Catalog Number: GRP13248
| Product Name | Anti-SARS-CoV-2 Nucleocapsid (N) Protein (RABBIT) Antibody |
|---|---|
| Catalog Number | GRP13248 |
| Species/Host | Rabbit |
| Reactivity | Virus |
| Conjugation | Unconjugated |
| Tested applications | ELISA, WB |
| Immunogen | Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells. |
| Alternative Names | (click to expand) |
| Form/Appearance | Liquid (sterile filtered) |
|---|---|
| Concentration | 1.0 mg/mL |
| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | This affinity purified antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein. The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 resin. BLAST analysis was used to suggest reactivity with related Coronavirus proteins. Cross reactivity with homologues from other sources has not been determined. |
SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2 or COVID-19) is related to SARS-CoV, MERS, and four milder coronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive-strand RNA virus that consists of four structural proteins: spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein. The spike protein is the most important surface protein of coronavirus. SARS-CoV-2 has a high affinity binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial. ACE2 is a membrane-bound aminopeptidase that has a vital role in the cardiovascular and immune systems. The nucleocapsid protein is a most abundant protein of coronavirus. The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics and viral research.
| Dilution Range | 1:500,000-1:600,000 |
|---|---|
| Application Notes | Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. |
