ALDH1A3 antibody [N2C2], Internal

Catalog Number: GRP128

Product Overview

Product Name	ALDH1A3 antibody [N2C2], Internal
Catalog Number	GRP128
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-P, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.
Alternative Names	(click to expand) aldehyde dehydrogenase 1 family member A3 \| ALDH1A6 \| ALDH6 \| MCOP8 \| RALDH3

Product Properties

Form/Appearance	Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P47895
Entrez	220

Product Description

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000

Validation Images

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GRP580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GRP580) diluted at 1:2000.

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GRP580) diluted at 1:50

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat prostate.ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GRP580) diluted at 1:500.Antigen Retrieval: Citrate buffer,

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate.ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GRP580) diluted at 1:500.Antigen Retrieval: Citrate buffe

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