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Product Overview
| Product Name | ALDH1A2 antibody [C1C3] |
|---|---|
| Catalog Number | GRP146 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALDH1A2. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.43 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | O94788 |
| Entrez | 8854 |
Product Description
This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
![ALDH1A2 antibody [C1C3] detects ALDH1A2 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ALDH1A2 antibody [C1C3] (GRP598) diluted at 1:500.](https://www.grp-ak.de/media/catalog/product/a/l/aldh1a2-antibody-c1c3_grp598_ihc-p_1_2.jpg)
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody (GRP598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ALDH1A2 antibody detects ALDH1A2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody (GRP598) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody wa
ALDH1A2 antibody [C1C3] detects ALDH1A2 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ALDH1A2 antibody [C1C3] (GRP598) diluted at 1:500.
Sample (30 μg of whole cell lysate) A: IMR32 10% SDS PAGE GRP598 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (50 μg of whole cell lysate) A: mouse liver 7.5% SDS PAGE GRP598 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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