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Product Overview
| Product Name | AKT (phospho Ser473) antibody |
|---|---|
| Catalog Number | GRP71 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, IP, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human AKT (phospho Ser473). The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.06 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P31749 |
| Entrez | 207 |
Product Description
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GRP523) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the
Untreated (–) and treated (+) HUVEC whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GRP523) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detec
MCF-7 cells were untreated or treated with 500 nM PMA for 1, 2, 4 and 6 hrs. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GRP523) diluted at 1:500. The HRP-conjuga
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GRP523) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to det
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GRP523) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detec
AKT (phospho Ser473) antibody detects AKT (phospho Ser473) protein by immunofluorescent analysis.Sample: NIH3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AKT (phospho Ser473) stained by AKT (phospho Ser473) antibody (GRP523) diluted
The WB analysis of AKT (phospho Ser473) antibody was published by Sun W and colleagues in the journal Cell Death Dis in 2014 .
The WB analysis of AKT (phospho Ser473) antibody was published by Vallejo-Flores G and colleagues in the journal Biomed Res Int in 2015.PMID: 26557697
The WB analysis of AKT (phospho Ser473) antibody was published by Lin JG and colleagues in the journal Int J Mol Sci in 2017.PMID: 28837077
Immunoprecipitation of Akt1/2/3 (phospho Ser473/474/472) protein from 293T whole cell extracts using 5 ?g of Akt1/2/3 (phospho Ser473/474/472) antibody (GRP523) antibody (GRP523).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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