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AKT antibody [N3C2], Internal

Catalog Number: GRP61

Product Overview
Product Name AKT antibody [N3C2], Internal
Catalog Number GRP61
Species/Host Rabbit
Reactivity Human, Mouse, Rat, Fish
Conjugation Unconjugated
Tested applications ICC, IF, IHC-Fr, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human Akt1/2/3. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.3 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Product Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000IP: 1:100-1:500
Validation Images
Sample (30 μg of whole cell lysate) A: PC-12 7.5% SDS PAGE GRP513 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Staining of Human normal esophagus tissue sections using anti-Akt1/2/3 antibody (GRP513).
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GRP513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used
Sample (30 μg of whole cell lysate) A: JC B: BCL-1 7.5% SDS PAGE GRP513 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GRP513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and t
AKT antibody [N3C2], Internal detects AKT protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AKT stained by AKT antibody [N3C2], Internal (GRP513) diluted at 1:500.Blue: Hoechst
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GRP513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
The WB analysis of AKT antibody [N3C2], Internal was published by Sun W and colleagues in the journal Cell Death Dis in 2014 .
The WB analysis of AKT antibody [N3C2], Internal was published by Vallejo-Flores G and colleagues in the journal Biomed Res Int in 2015.PMID: 26557697
Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 ?g of Akt1/2/3 antibody [N3C2], Internal (GRP513).Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GRP513).EasyBlot anti-Rabbit IgG was used as a s
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