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SQSTM1 / P62 antibody [N3C1], Internal

Catalog Number: GRP15

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Datasheet
Product Overview
Product Name SQSTM1 / P62 antibody [N3C1], Internal
Catalog Number GRP15
Species/Host Rabbit
Reactivity Human, Mouse, Rat, Bovine, Zebrafish, Honeybee
Conjugation Unconjugated
Tested applications FACS, ICC, IF, IHC-P, IP, WB
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human SQSTM1 / P62. The exact sequence is proprietary.
Alternative Names (click to expand) sequestosome 1 | A170 | DMRV | FTDALS3 | NADGP | OSIL | PDB3 | ZIP3 | p60 | p62 | p62B
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.29 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID Q13501
Entrez 8878
Product Description

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,FACS: 1:50-1:200,IP: 1:100-1:500
Validation Images
SQSTM1 antibody [N3C1], Internal (GRP467) detects SQSTM1 protein by flow cytometry analysis.Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min.Brown: Unlabelled sample was also used as a control.Blue: SQSTM1 antibody [N3C1], Internal] dilut
SQSTM1 antibody [N3C1], Internal (GRP467) detects SQSTM1 protein by flow cytometry analysis.Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min.Brown: Unlabelled sample was also used as a control.Blue: SQSTM1 antibody [N3C1], Internal] dilut
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50?M Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: SQSTM1 pr
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50?M Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: SQSTM1 pr
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GRP467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to de
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GRP467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to de
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract10% SDS-PAGESQSTM1 antibody [N3C1], Internal (GRP467) dilution: 1:1000 The HRP-conjugate
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract10% SDS-PAGESQSTM1 antibody [N3C1], Internal (GRP467) dilution: 1:1000 The HRP-conjugate
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.Samples: HepG2 cells treated with 3?M thapsigargin 12 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acet
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.Samples: HepG2 cells treated with 3?M thapsigargin 12 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acet
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.A. 30 μg NIH-3T3 whole cell lysate/extract B. 30 μg JC whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract 12% SDS-PAGESQSTM1 antibody [N3C1], Interna
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.A. 30 μg NIH-3T3 whole cell lysate/extract B. 30 μg JC whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract 12% SDS-PAGESQSTM1 antibody [N3C1], Interna
Untreated (–) and treated (+) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GRP467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to de
Untreated (–) and treated (+) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GRP467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to de
SQSTM1 / P62 antibody [N3C1], Internal detects SQSTM1 / P62 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.SQSTM1 / P62 stained by SQSTM1 / P62 antibody [N3C1], Internal (GRP467) diluted at 1:500.Antigen R
SQSTM1 / P62 antibody [N3C1], Internal detects SQSTM1 / P62 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.SQSTM1 / P62 stained by SQSTM1 / P62 antibody [N3C1], Internal (GRP467) diluted at 1:500.Antigen R
Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 ?g of SQSTM1 antibody [N3C1], Internal (GRP467).Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal (GRP467).EasyBlot anti-Rabbit IgG was used as a seconda
Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 ?g of SQSTM1 antibody [N3C1], Internal (GRP467).Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal (GRP467).EasyBlot anti-Rabbit IgG was used as a seconda
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